Coding

Part:BBa_K3041001

Designed by: Melanie Ofman   Group: iGEM19_Leiden   (2019-10-07)


Suckerin-9


Coding gene of suckerin-9 from the Humboldt squid Dosidicus gigas, codon optimized for expression in E. coli.

Validation

The successfully synthesized suckerins, codon-optimized for E.coli, were PCR amplified using the standard biobrick primers. In Figure 1, the PCR products of these specific suckerins were visualized on agarose gel. Suckerin-9, shows strong bands on agarose gel.


PCR suckerin proteins.png


Figure 1. Amplified suckerin genes 8, 9, and 12. Polymerase chain reaction (PCR) products amplified with general prefix and suffix primers, shown by gel electrophoresis. Lane 1: 1kb ladder. PCR product at different concentrations of suckerin-8 (408 bp, lane 2 and 3), suckerin-9 (568 bp, lane 4 and 5), and suckerin-12 (696 bp, lane 6 and 7). The gel confirms the amplification of the desired suckerin proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None